Ben Te’o and Mike Brown have been omitted from England‘s 31-man World Cup squad by Eddie Jones.
Former captain Dylan Hartley is another high-profile player to be cut.
Te’o has lost out to Piers Francis among the four centres bound for Japan 2019 despite being an ever-present under Jones when available.
The 32-year-old was involved in a scuffle with Brown during a social event at the squad’s training camp in Treviso a fortnight ago.
There was also no place in the travelling party for Chris Ashton, Chris Robshaw or Danny Care.
Jones said over the weekend that Te’o was still in World Cup contention but the Australian has chosen to leave him out.
Former sevens specialist Ruaridh McConnochie continues a remarkable year by being present as the only uncapped player on the strength of a strong end to the season for Bath.
Flanker Lewis Ludlam, hooker Jack Singleton and scrum-half Willi Heinz made their debuts in Sunday’s rout of Wales and are also picked.
England’s World Cup squad
Dan Cole (Leicester Tigers), Luke Cowan-Dickie (Exeter Chiefs), Tom Curry (Sale Sharks), Ellis Genge (Leicester Tigers), Jamie George (Saracens), Maro Itoje (Saracens), George Kruis (Saracens), Joe Launchbury (Wasps), Courtney Lawes (Northampton Saints), Lewis Ludlam (Northampton Saints), Joe Marler (Harlequins), Kyle Sinckler (Harlequins), Jack Singleton (Saracens), Sam Underhill (Bath Rugby), Billy Vunipola (Saracens), Mako Vunipola (Saracens), Mark Wilson (Newcastle Falcons/Sale Sharks)
Joe Cokanasiga (Bath Rugby), Elliot Daly (Saracens), Owen Farrell (Saracens), George Ford (Leicester Tigers), Piers Francis (Northampton Saints), Willi Heinz (Gloucester Rugby), Jonathan Joseph (Bath Rugby), Jonny May (Leicester Tigers), Ruaridh McConnochie (Bath Rugby), Jack Nowell (Exeter Chiefs), Henry Slade (Exeter Chiefs), Manu Tuilagi (Leicester Tigers), Anthony Watson (Bath Rugby), Ben Youngs (Leicester Tigers)
Jones has gambled by taking only two scrum-halves with Heinz providing cover for Ben Youngs, who is first choice in the position.
The decision to pick just five props means Harry Williams misses out, with Kyle Sinckler and Dan Cole travelling as the two tightheads.
Brad Shields will also miss out on the World Cup as he continues his rehabilitation from the foot injury incurred in Treviso.
The outlook on Shields left him with a race against time to board the plane with the rest of the squad on September 8 and Ludlam has been chosen in his place.